Rat IL-10 High Sensitivity ELISA Kit
SKU: 94008934307

Rat IL-10 High Sensitivity ELISA Kit

Sale price$202.50 Regular price$225.00
Save 10%

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Jul 5 - Jul 10

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

Rat IL-10 High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti rat IL 10 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL 10 in the sample binds to the solid phase antibody. After

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti rat IL-10 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-10 in the sample binds to the solid-phase antibody. After thorough washing to remove free and unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-10 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: a copy of pre coated 96 well plate, standard, biotin labeled IL-10 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.22 pg/ml

Detection Range: 6.25-400 pg/ml

Recovery Range: 94-115%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 10 (IL-10) is a homodimer composed of two 178 amino acid subunits. In humans, it is encoded by the IL-10 gene located on chromosome 1 and consists of five exons. IL10 is mainly secreted by monocytes, lymphocytes, type II helper T cells (Th2), mast cells, cd4+cd25+foxp3+ regulatory T cells and partially activated T cells and B cells secreted less. It has multiple effects on immune regulation and inflammation. It downregulates Th1 cytokines, MHC class II antigens and costimulatory molecules of macrophages. It can also enhance B cell survival, proliferation and antibody secretion. IL-10 can block NF - & kappa; B activity, and participate in JAK-STAT signaling pathway regulation. The immunosuppressive properties of IL-10 suggest that it may be used in clinic to inhibit rejection after organ transplantation. IL-10 also has strong anti-inflammatory properties.

Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 94008934307

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.4 ★★★★★
Based on 501 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
A
Verified Purchase
Alex Billeter
Fort Morgan, US
★★★★★ 5
Great little snuffle toy
Color: Squirrel
Our dogs love the squirrel! Great little interactive snuffle toy. Better for small dogs imo but our medium dog loves it too.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 16, 2025
T
Verified Purchase
Traci Butterfield
Louisville, US
★★★★★ 5
Great busy toy!
Color: Squirrel
My dog loves this toy! And I hate squirrels, so I love to see him try to k*ll it. Haha Some of the "pockets" are a little deep and he did manage to rip 1 pocket off during the first use; but it's still keeping him busy!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 5, 2025
J
Verified Purchase
Jennifer Mak
West Palm Beach, US
★★★★★ 5
Velcro for folding?
The toy came folded in a bag very nice. However once I took it out I realized the folds were attached by Velcro. Once unrolled I have not needed to use the Velcro since. Just one piece of the toy that was unnecessary. Otherwise t the dot is very colorful and my dog has not been able to tear it up yet.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 27, 2024
C
Verified Purchase
Carley
Lake Worth, US
★★★★★ 4
Great toy for moderately destructive dogs
Color: Dragon
My dog LOVES this toy. It's not hard to see why: it has just about everything a dog could want in a soft toy. There are ropes that move around, a squeaker, crinkly wings, and different textures of fabric. It's not extremely durable--I've had to sew up one busted seam within a month or so--and you will likely have to re-knot the ropes and pull them back through the holes, but if your dog is only moderately destructive, they will enjoy this. I'd buy another.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 11, 2025
S
Verified Purchase
S. Hart
Boise, US
★★★★★ 3
Dog Obsessed -- Toy a Mess
Color: Squirrel
The dog was OBSESSED. Truly loved this toy, but he ripped the little pockets really easily. While he still wanted to play with it, it was a little disappointing.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 28, 2026

recommand products